|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human CRELD1 ORF mammalian expression plasmid, C-GFPSpark tag||HG13411-ACG|
|Human CRELD1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13411-ACR|
|Human CRELD1 ORF mammalian expression plasmid, C-Flag tag||HG13411-CF|
|Human CRELD1 ORF mammalian expression plasmid, C-His tag||HG13411-CH|
|Human CRELD1 ORF mammalian expression plasmid, C-Myc tag||HG13411-CM|
|Human CRELD1 ORF mammalian expression plasmid, C-HA tag||HG13411-CY|
|Human CRELD1 Gene cDNA clone plasmid||HG13411-G|
|Human CRELD1 ORF mammalian expression plasmid, N-Flag tag||HG13411-NF|
|Human CRELD1 ORF mammalian expression plasmid, N-His tag||HG13411-NH|
|Human CRELD1 ORF mammalian expression plasmid, N-Myc tag||HG13411-NM|
|Human CRELD1 ORF mammalian expression plasmid, N-HA tag||HG13411-NY|
|Human CRELD1 natural ORF mammalian expression plasmid||HG13411-UT|
|Learn more about expression Vectors|
CRELD1 is a transmembrane glycoprotein. Epidermal growth factor(EGF)like domain exists in CRELD1. EGF-like repeats are a class of cysteine-rich domains that mediate interactions between proteins of diverse function. EGF domains are found in proteins that are either completely secreted or have transmembrane regions that tether the protein to the cell surface. CRELD1 contains a 333 amino acid acid (aa) extracellular domain (ECD), two tandem transmembrane segments, and a second ECD of 15 aa. Defects in CRELD1 may cause susceptibility to atrioventricular septal defect type 2 which results in a persistent common atrioventricular canal.