|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human FSTL5 ORF mammalian expression plasmid, C-GFPSpark tag||HG13408-ACG|
|Human FSTL5 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13408-ACR|
|Human FSTL5 ORF mammalian expression plasmid, C-Flag tag||HG13408-CF|
|Human FSTL5 ORF mammalian expression plasmid, C-His tag||HG13408-CH|
|Human FSTL5 ORF mammalian expression plasmid, C-Myc tag||HG13408-CM|
|Human FSTL5 ORF mammalian expression plasmid, C-HA tag||HG13408-CY|
|Human FSTL5 Gene cDNA clone plasmid||HG13408-G|
|Human FSTL5 ORF mammalian expression plasmid, N-Flag tag||HG13408-NF|
|Human FSTL5 ORF mammalian expression plasmid, N-His tag||HG13408-NH|
|Human FSTL5 ORF mammalian expression plasmid, N-Myc tag||HG13408-NM|
|Human FSTL5 ORF mammalian expression plasmid, N-HA tag||HG13408-NY|
|Human FSTL5 natural ORF mammalian expression plasmid||HG13408-UT|
|Learn more about expression Vectors|
FSTL5 may have molecular function (calcium ion binding) and to localize in various compartments (cytoplasm, extracellular space, extracellular region). FSTL5 expression denoted a dismal prognosis both within and across medulloblastoma subgroups. FSTL5 gene is well expressed, 1.0 times the average gene in this release. The sequence of this gene is defined by 120 GenBank accessions from 113 cDNA clones, some from brain, cerebellum, eye, melanotic melanoma, skin, amygdala, breast and 24 other tissues. FSTL5 gene contains 27 distinct introns. The addition of FSTL5 immunohistochemistry to existing molecular stratification schemes constitutes a reliable and cost-effective tool for prognostication in future clinical trials of medulloblastoma.