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Cynomolgus PDGFB / PDGF-BB Protein (Fc Tag)

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Cynomolgus PDGFB Protein Product Information
Synonym:PDGFB
Protein Construction:A DNA sequence encoding the cynomolgus PDGFB (EHH65851.1) (Ser82-Thr190) was expressed with the Fc region of mouse IgG1 at the N-terminus.
Species:Cynomolgus
Expressed Host:Human Cells
Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Cynomolgus PDGFB Protein QC Testing
Purity:> 95 % as determined by SDS-PAGE
Bio-Activity:1. Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 5-25 ng/ml.
2. Measured by its binding ability in a functional ELISA. Immobilized Cynomolgus PDGFRB-His (Cat:90215-C08H) at 10 μg/ml (100 μl/well) can bind Cynomolgus S4-mFc3-PDGFB, The EC50 of Cynomolgus S4-mFc3-PDGFB is 3.6-7.4 ng/ml.
Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Asp
Molecule Mass:The recombinant cynomolgus PDGFB is a disulfide-linked homodimer. The reduced monomer comprises 345 amino acids and has a calculated molecular mass of 38.9 KDa. The apparent molecular mass of it is approximately 43 and 32 KDa in SDS-PAGE.
Formulation:Lyophilized from sterile PBS, pH 7.4.
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Cynomolgus PDGFB Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Other PDGFB Recombinant Protein Products
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Catalog: 90030-C04H-10
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"