|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human PNLIP ORF mammalian expression plasmid, C-GFPSpark tag||HG13564-ACG|
|Human PNLIP ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13564-ACR|
|Human PNLIP ORF mammalian expression plasmid, C-Flag tag||HG13564-CF|
|Human PNLIP ORF mammalian expression plasmid, C-His tag||HG13564-CH|
|Human PNLIP ORF mammalian expression plasmid, C-Myc tag||HG13564-CM|
|Human PNLIP ORF mammalian expression plasmid, C-HA tag||HG13564-CY|
|Human PNLIP Gene cDNA clone plasmid||HG13564-G|
|Human PNLIP ORF mammalian expression plasmid, N-Flag tag||HG13564-NF|
|Human PNLIP ORF mammalian expression plasmid, N-His tag||HG13564-NH|
|Human PNLIP ORF mammalian expression plasmid, N-Myc tag||HG13564-NM|
|Human PNLIP ORF mammalian expression plasmid, N-HA tag||HG13564-NY|
|Human PNLIP natural ORF mammalian expression plasmid||HG13564-UT|
|Learn more about expression Vectors|
PNLIP is an enzyme which belongs to the lipase family. Secreted from the pancreas, PNLIP is the primary lipase that hydrolyzes dietary fat molecules in the human digestive system, converting triglyceride substrates found in ingested oils to monoglycerides and free fatty acids. Bile salts secreted from the liver and stored in gallbladder are released into the duodenum where they coat and emulsify large fat droplets into smaller droplets, thus increasing the overall surface area of the fat, which allows the lipase to break apart the fat more effectively. The resulting monomers (2 free fatty acids and one 2-monoacylglycerol) are then moved by way of peristalsis along the small intestine to be absorbed into the lymphatic system by a specialized vessel called a lacteal.