|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human PHYH ORF mammalian expression plasmid, C-GFPSpark tag||HG13368-ACG|
|Human PHYH ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13368-ACR|
|Human PHYH ORF mammalian expression plasmid, N-GFPSpark tag||HG13368-ANG|
|Human PHYH ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG13368-ANR|
|Human PHYH ORF mammalian expression plasmid, C-Flag tag||HG13368-CF|
|Human PHYH ORF mammalian expression plasmid, C-His tag||HG13368-CH|
|Human PHYH ORF mammalian expression plasmid, C-Myc tag||HG13368-CM|
|Human PHYH ORF mammalian expression plasmid, C-HA tag||HG13368-CY|
|Human PHYH Gene cDNA clone plasmid||HG13368-G|
|Human PHYH ORF mammalian expression plasmid, N-Flag tag||HG13368-NF|
|Human PHYH ORF mammalian expression plasmid, N-His tag||HG13368-NH|
|Human PHYH ORF mammalian expression plasmid, N-Myc tag||HG13368-NM|
|Human PHYH ORF mammalian expression plasmid, N-HA tag||HG13368-NY|
|Human PHYH natural ORF mammalian expression plasmid||HG13368-UT|
|Learn more about expression Vectors|
PHYH belongs to the family of iron(II)-dependent oxygenases, which typically incorporate one atom of dioxygen into the substrate and one atom into the succinate carboxylate group. PHYH is expressed in liver, kidney, and T-cells, but not in spleen, brain, heart, lung and skeletal muscle. It converts phytanoyl-CoA to 2-hydroxyphytanoyl-CoA. Defects in PHYH can cause Refsum disease (RD). RD is an autosomal recessive disorder characterized clinically by a tetrad of abnormalities: retinitis pigmentosa, peripheral neuropathy, cerebellar ataxia, and elevated protein levels in the cerebrospinal fluid (CSF). Patients exhibit accumulation of the branched-chain fatty acid, phytanic acid, in blood and tissues.