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CD146 / MCAM Antibody (PE), Rabbit MAb

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MCAMAntibody Product Information
Immunogen:Recombinant Human CD146 protein (Catalog#10115-H08H)
Clone ID:044
Ig Type:Rabbit IgG
Concentration:2 μl/Test, 0.1 mg/ml
Formulation:Aqueous solution containing 0.5% BSA and 0.1% sodium azide
Preparation:This antibody was obtained from a rabbit immunized with purified, recombinant Human CD146 / MCAM (rh CD146; Catalog#10115-H08H; NP_006491.2; Met 1-Gly 559) and conjugated with PE under optimum conditions, the unreacted PE was removed.
MCAMAntibody Usage Guide
Specificity:Human CD146 / MCAMNo cross-reactivity in ELISA with
Human CD66a
Human CD166
Human CD106
Human BCAM
Human CD226
Application:ICC/IF, FCM, IF

ICC/IF: 5-20 μg/mL

Storage:This antibody is stable for 12 months from date of receipt when stored at 2℃-8℃. Protected from prolonged exposure to light. Do not freeze !
Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.
CD146 / MCAM Antibody (PE), Rabbit MAb, Flow cytometric
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Profile of anti-CD146 reactivity on HeLa cells analyzed by flow cytometry.
CD146 / MCAM Antibody (PE), Rabbit MAb, Immunofluorescence
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Immunofluorescence staining of Human CD146 in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human CD146 monoclonal antibody (PE-conjugated, 10 µg/ml) at 4℃ overnight, and counterstained with DAPI (blue). Positive staining was localized to plasma membrane.

The CD146 antigen, also known as melanoma cell adhesion molecule (MCAM) and MUC18, is an integral membrane glycoprotein belonging to the immunoglobulin superfamily. CD146 contains the characteristic immunoglobulin-like domains (V-V-C2-C2-C2), a transmembrane region and a short cytoplasmic tail. The CD146 expression is detected in endothelial cells in vascular tissue throughout the body, and plays a role in cell adhesion, as well as in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. As a Ca2+-independent cell adhesion molecule involved in heterophilic cell to cell interactions and a surface receptor, CD146 triggers tyrosine phosphorylation of FYN and PTK2 and subsequently induced signal transduction, proteolysis, or immune recognition. This protein is also expressed predominantly on metastatic lesions and advanced primary tumours, and thus has been suggested to play an important role in tumour progression and the development of metastasis in certain human carcinomas.

  • Mills L, et al. (2002) Fully human antibodies to MCAM/MUC18 inhibit tumor growth and metastasis of human melanoma. Cancer Res. 62(17): 5106-14.
  • Taira E, et al. (2004) Characterization of Gicerin/MUC18/CD146 in the rat nervous system. J Cell Physiol. 198(3): 377-87.
  • Fritzsche FR, et al. (2008) CD146 protein in prostate cancer: revisited with two different antibodies. Pathology. 40(5): 457-64.
  • Bidlingmaier S, et al. (2009) Identification of MCAM/CD146 as the target antigen of a human monoclonal antibody that recognizes both epithelioid and sarcomatoid types of mesothelioma. Cancer Res. 69(4): 1570-7.
  • Boneberg EM, et al. (2009) Soluble CD146 is generated by ectodomain shedding of membrane CD146 in a calcium-induced, matrix metalloprotease-dependent process. Microvasc Res. 78(3): 325-31.
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    • CD146 / MCAM Antibody (PE), Rabbit MAb, Flow cytometric
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