|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human GM2A ORF mammalian expression plasmid, C-GFPSpark tag||HG13246-ACG|
|Human GM2A ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13246-ACR|
|Human GM2A ORF mammalian expression plasmid, C-Flag tag||HG13246-CF|
|Human GM2A ORF mammalian expression plasmid, C-His tag||HG13246-CH|
|Human GM2A ORF mammalian expression plasmid, C-Myc tag||HG13246-CM|
|Human GM2A ORF mammalian expression plasmid, C-HA tag||HG13246-CY|
|Human GM2A Gene cDNA clone plasmid||HG13246-G|
|Human GM2A ORF mammalian expression plasmid, N-Flag tag||HG13246-NF|
|Human GM2A ORF mammalian expression plasmid, N-His tag||HG13246-NH|
|Human GM2A ORF mammalian expression plasmid, N-Myc tag||HG13246-NM|
|Human GM2A ORF mammalian expression plasmid, N-HA tag||HG13246-NY|
|Human GM2A natural ORF mammalian expression plasmid||HG13246-UT|
|Learn more about expression Vectors|
GM2A (GM2 ganglioside activator), is a lipid transfer protein which belongs to the ML domain family. GM2A can accommodate several single chain phospholipids and fatty acids. It also exhibits some calcium-independent phospholipase activity. GM2A binds gangliosides and stimulates ganglioside GM2 degradation. It stimulates only the breakdown of ganglioside GM2 and glycolipid GA2 by beta-hexosaminidase A. GM2A acts as a substrate specific co-factor for the lysosomal enzyme β-hexosaminidase A. β-hexosaminidase A, together with GM2 ganglioside activator, catalyzes the degradation of the ganglioside GM2, and other molecules containing terminal N-acetyl hexosamines. It extracts single GM2 molecules from membranes and presents them in soluble form to beta-hexosaminidase A for cleavage of N-acetyl-D-galactosamine and conversion to GM3. Defects in GM2A are the cause of GM2-gangliosidosis type AB (GM2GAB), also known as Tay-Sachs disease AB variant.