|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human IL1RN / IL1F3 ORF mammalian expression plasmid, C-GFPSpark tag||HG13245-ACG|
|Human IL1RN / IL1F3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13245-ACR|
|Human IL1RN / IL1F3 ORF mammalian expression plasmid, C-Flag tag||HG13245-CF|
|Human IL1RN / IL1F3 ORF mammalian expression plasmid, C-His tag||HG13245-CH|
|Human IL1RN / IL1F3 ORF mammalian expression plasmid, C-Myc tag||HG13245-CM|
|Human IL1RN / IL1F3 ORF mammalian expression plasmid, C-HA tag||HG13245-CY|
|Human IL1RN / IL1F3 Gene cDNA clone plasmid||HG13245-G|
|Human IL1RN / IL1F3 ORF mammalian expression plasmid, N-Flag tag||HG13245-NF|
|Human IL1RN / IL1F3 ORF mammalian expression plasmid, N-His tag||HG13245-NH|
|Human IL1RN / IL1F3 ORF mammalian expression plasmid, N-Myc tag||HG13245-NM|
|Human IL1RN / IL1F3 ORF mammalian expression plasmid, N-HA tag||HG13245-NY|
|Human IL1RN / IL1F3 natural ORF mammalian expression plasmid||HG13245-UT|
|Learn more about expression Vectors|
Interleukin-1 receptor antagonist (IL-1RA) also known as IL1RN is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. A polymorphism of this protein encoding gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. IL-1RA/IL1RN may inhibit the activity of IL-1 by binding to its receptor and it has no IL-1 like activity. Genetic variation in IL-1RA/IL1RN is associated with susceptibility to microvascular complications of diabetes type 4 (MVCD4). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Defects in IL-1RA/IL1RN are the cause of interleukin 1 receptor antagonist deficiency (DIRA) which is also known as deficiency of interleukin 1 receptor antagonist. Autoinflammatory diseases manifest inflammation without evidence of infection, high-titer autoantibodies, or autoreactive T-cells. DIRA is a rare, autosomal recessive, genetic autoinflammatory disease that results in sterile multifocal osteomyelitis, and pustulosis from birth.