|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human AZGP1 ORF mammalian expression plasmid, C-GFPSpark tag||HG13242-ACG|
|Human AZGP1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13242-ACR|
|Human AZGP1 ORF mammalian expression plasmid, C-Flag tag||HG13242-CF|
|Human AZGP1 ORF mammalian expression plasmid, C-His tag||HG13242-CH|
|Human AZGP1 ORF mammalian expression plasmid, C-Myc tag||HG13242-CM|
|Human AZGP1 ORF mammalian expression plasmid, C-HA tag||HG13242-CY|
|Human AZGP1 Gene cDNA clone plasmid||HG13242-G|
|Human AZGP1 ORF mammalian expression plasmid, N-Flag tag||HG13242-NF|
|Human AZGP1 ORF mammalian expression plasmid, N-His tag||HG13242-NH|
|Human AZGP1 ORF mammalian expression plasmid, N-Myc tag||HG13242-NM|
|Human AZGP1 ORF mammalian expression plasmid, N-HA tag||HG13242-NY|
|Human AZGP1 natural ORF mammalian expression plasmid||HG13242-UT|
|Learn more about expression Vectors|
Alpha-2-glycoprotein, also known as AZGP1, belongs to the MHC class I family. It can be detected in body fluids such as serum, sweat, and seminal and breast cyst fluids. It has been shown that alpha-2-glycoprotein can stimulate lipolysis by adipocytes in vivo and in vitro. Thus it is believed that alpha-2-glycoprotein plays an important role in the regulation of body weight, and age-dependent changes in genetically influenced obesity, and it also regulates melanin production by normal and malignant melanocytes. Alpha-2-glycoprotein is produced by both white and brown fat adipocytes and may act in a local autocrine fashion in the reduction of adiposity in cachexia.