|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human SEMA5A ORF mammalian expression plasmid, C-GFPSpark tag||HG11300-ACG|
|Human SEMA5A ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11300-ACR|
|Human SEMA5A ORF mammalian expression plasmid, C-Flag tag||HG11300-CF|
|Human SEMA5A ORF mammalian expression plasmid, C-His tag||HG11300-CH|
|Human SEMA5A ORF mammalian expression plasmid, C-Myc tag||HG11300-CM|
|Human SEMA5A ORF mammalian expression plasmid, C-HA tag||HG11300-CY|
|Human SEMA5A Gene cDNA clone plasmid||HG11300-G|
|Human SEMA5A ORF mammalian expression plasmid, N-Flag tag||HG11300-NF|
|Human SEMA5A ORF mammalian expression plasmid, N-His tag||HG11300-NH|
|Human SEMA5A ORF mammalian expression plasmid, N-Myc tag||HG11300-NM|
|Human SEMA5A ORF mammalian expression plasmid, N-HA tag||HG11300-NY|
|Human SEMA5A natural ORF mammalian expression plasmid||HG11300-UT|
|Learn more about expression Vectors|
Semaphorins are secreted, transmembrane, and GPI-linked proteins, defined by cysteine-rich semaphorin protein domains, that have important roles in a variety of tissues. Humans have 20 semaphorins, Drosophila has five, and two are known from DNA viruses. Semaphorins are found in nematodes and crustaceans but not in non-animals. They are grouped into eight classes on the basis of phylogenetic tree analyses and the presence of additional protein motifs. Semaphorins have been implicated in diverse developmental processes such as axon guidance during nervous system development and regulation of cell migration. Semaphorin-5A, also known as Semaphorin-F, Sema F, SEMA5A and SEMAF, is a single-pass type I membrane protein which belongs to the semaphorin family. Semaphorin5A / SEMA5A contains one PSI domain, one Sema domain and seven TSP type-1 domains. It may act as positive axonal guidance cues. Semaphorin5A / SEMA5A is an axon regulator molecule and plays major roles during neuronal and vascular development. It plays an essential role in embryonic development. Semaphorin5A / SEMA5A induces endothelial cell migration from pre-existing vessels. It also plays a role in autism, reducing the ability of neurons to form connections with other neurons in certain brain regions.