|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human TYRP1 ORF mammalian expression plasmid, C-GFPSpark tag||HG13224-ACG|
|Human TYRP1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13224-ACR|
|Human TYRP1 ORF mammalian expression plasmid, C-Flag tag||HG13224-CF|
|Human TYRP1 ORF mammalian expression plasmid, C-His tag||HG13224-CH|
|Human TYRP1 ORF mammalian expression plasmid, C-Myc tag||HG13224-CM|
|Human TYRP1 ORF mammalian expression plasmid, C-HA tag||HG13224-CY|
|Human TYRP1 Gene cDNA clone plasmid||HG13224-G|
|Human TYRP1 ORF mammalian expression plasmid, N-Flag tag||HG13224-NF|
|Human TYRP1 ORF mammalian expression plasmid, N-His tag||HG13224-NH|
|Human TYRP1 ORF mammalian expression plasmid, N-Myc tag||HG13224-NM|
|Human TYRP1 ORF mammalian expression plasmid, N-HA tag||HG13224-NY|
|Human TYRP1 natural ORF mammalian expression plasmid||HG13224-UT|
|Learn more about expression Vectors|
Tyrosinase-related protein 1, also known as TYRP1 or TRP1, is a melanosomal enzyme that belongs to the tyrosinase family and plays an important role in the melanin biosynthetic pathway. Mutations in this enzyme are the cause of rufous oculocutaneous albinism and oculocutaneous albinism type III. TYRP1 / TRP1 is involved in the oxidation of 5,6-dihydroxyindole-2-carboxylic acid (DHICA) into indole-5,6-quinone-2-carboxylic acid. This enzyme may regulate or influence the type of melanin synthesized. The expression of Tyrosinase-related protein 1 (TYRP1) is regulated by the microphthalmia-associated transcription factor (MITF). There is mounting evidence demonstrating that in addition to its role in eumelanin synthesis, TYRP1 is involved in maintaining stability of tyrosinase proliferation and melanocyte cell death.