|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human STAT6 ORF mammalian expression plasmid, C-GFPSpark tag||HG13190-ACG|
|Human STAT6 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13190-ACR|
|Human STAT6 ORF mammalian expression plasmid, N-GFPSpark tag||HG13190-ANG|
|Human STAT6 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG13190-ANR|
|Human STAT6 ORF mammalian expression plasmid, C-Flag tag||HG13190-CF|
|Human STAT6 ORF mammalian expression plasmid, C-His tag||HG13190-CH|
|Human STAT6 ORF mammalian expression plasmid, C-Myc tag||HG13190-CM|
|Human STAT6 ORF mammalian expression plasmid, C-HA tag||HG13190-CY|
|Human STAT6 Gene cDNA clone plasmid||HG13190-G|
|Human STAT6 ORF mammalian expression plasmid, N-Flag tag||HG13190-NF|
|Human STAT6 ORF mammalian expression plasmid, N-His tag||HG13190-NH|
|Human STAT6 ORF mammalian expression plasmid, N-Myc tag||HG13190-NM|
|Human STAT6 ORF mammalian expression plasmid, N-HA tag||HG13190-NY|
|Human STAT6 natural ORF mammalian expression plasmid||HG13190-UT|
|Learn more about expression Vectors|
Signal transducer and activator of transcription 6 (STAT6) is a transcription factor that is activated by interleukin-4 (IL-4)-induced tyrosine phosphorylation and mediates most of the IL-4-induced gene expression. STAT6 plays a central role in exerting interleukin-4 (IL-4) mediated biological responses and is found to induce the expression of BCL2L1/BCL-XL, which is responsible for the anti-apoptotic activity of IL4. Transcriptional activation by STAT6 requires the interaction with coactivators like p300 and the CREB-binding protein (CBP). NF-?B and tyrosine-phosphorylated Stat6 can directly bind each other in vitro and in vivo, which suggest that the direct interaction between Stat6 and NF-?B may provide a basis for synergistic activation of transcription by IL-4 and activators of NF-?B.