|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human ANTXR1 ORF mammalian expression plasmid, C-GFPSpark tag||HG13367-ACG|
|Human ANTXR1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13367-ACR|
|Human ANTXR1 ORF mammalian expression plasmid, C-Flag tag||HG13367-CF|
|Human ANTXR1 ORF mammalian expression plasmid, C-His tag||HG13367-CH|
|Human ANTXR1 ORF mammalian expression plasmid, C-Myc tag||HG13367-CM|
|Human ANTXR1 ORF mammalian expression plasmid, C-HA tag||HG13367-CY|
|Human ANTXR1 Gene cDNA clone plasmid||HG13367-G|
|Human ANTXR1 ORF mammalian expression plasmid, N-Flag tag||HG13367-NF|
|Human ANTXR1 ORF mammalian expression plasmid, N-His tag||HG13367-NH|
|Human ANTXR1 ORF mammalian expression plasmid, N-Myc tag||HG13367-NM|
|Human ANTXR1 ORF mammalian expression plasmid, N-HA tag||HG13367-NY|
|Human ANTXR1 natural ORF mammalian expression plasmid||HG13367-UT|
|Learn more about expression Vectors|
ANTXR1 contains 1 VWFA domain and belongs to the ATR family. ATR (Ataxia telangiectasia and Rad3 related) and ATM (Ataxia telangiectasia mutated) are closely related kinases that are activated by DNA damage. They are serine-threonine protein kinases and belongs to the phosphatidylinositol 3' kinase-like kinase (PIKK) family. Upon recruitment by the DNA damage binding proteins/complexes (ATRIP for ATR; MRN for ATM), ATM/ATR initiate the DNA damage checkpoint by phosphorylating a number of key proteins. ANTXR1 interacts with extracellular matrix proteins and with the actin cytoskeleton. It functions in cell attachment and migration. ANTXR1 also mediates adhesion of cells to type 1 collagen and gelatin, reorganization of the actin cytoskeleton and promotes cell spreading. It plays a role in the angiogenic response of cultured umbilical vein endothelial cells.