|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human MKI67 ORF mammalian expression plasmid, C-GFPSpark tag||HG13180-ACG|
|Human MKI67 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13180-ACR|
|Human MKI67 ORF mammalian expression plasmid, N-GFPSpark tag||HG13180-ANG|
|Human MKI67 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG13180-ANR|
|Human MKI67 ORF mammalian expression plasmid, C-Flag tag||HG13180-CF|
|Human MKI67 ORF mammalian expression plasmid, C-His tag||HG13180-CH|
|Human MKI67 ORF mammalian expression plasmid, C-Myc tag||HG13180-CM|
|Human MKI67 ORF mammalian expression plasmid, C-HA tag||HG13180-CY|
|Human MKI67 Gene cDNA clone plasmid||HG13180-G|
|Human MKI67 ORF mammalian expression plasmid, N-Flag tag||HG13180-NF|
|Human MKI67 ORF mammalian expression plasmid, N-His tag||HG13180-NH|
|Human MKI67 ORF mammalian expression plasmid, N-Myc tag||HG13180-NM|
|Human MKI67 ORF mammalian expression plasmid, N-HA tag||HG13180-NY|
|Human MKI67 natural ORF mammalian expression plasmid||HG13180-UT|
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MKI67 contains 1 FHA domain and plays a key role in cell proliferation. During interphase, the MKI67 antigen can be exclusively detected within the cell nucleus, whereas in mitosis most of the protein is relocated to the surface of the chromosomes. MKI67 protein is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent from resting cells. MKI67 is an excellent marker to determine the growth fraction of a given cell population. The fraction of MKI67-positive tumor cells is often correlated with the clinical course of cancer. It is also associated with ribosomal RNA transcription. Inactivation of antigen MKI67 leads to inhibition of ribosomal RNA synthesis.
The MKI67 protein is a nuclear and nucleolar protein, which is tightly associated with somatic cell proliferation. Antibodies raised against the human MKI67 protein paved the way for the immunohistological assessment of cell proliferation, particularly useful in numerous studies on the prognostic value of cell growth in clinical samples of human neoplasms. MKI67 protein expression is an absolute requirement for progression through the cell-division cycle. Recently, MKI67 is proved be an independent prognostic factor for disease-free survival (HR 1.05-1.72) in multivariate analyses studies using samples from randomized clinical trials with secondary central analysis of the biomarker. MKI67 was not found to be predictive for long-term follow-up after chemotherapy. Nevertheless, high KI-67 was found to be associated with immediate pathological complete response in the neoadjuvant setting, with an LOE of II-B. MKI67 could be considered as a prognostic biomarker for therapeutic decision.