|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human HIST1H3A ORF mammalian expression plasmid, C-GFPSpark tag||HG11231-ACG|
|Human HIST1H3A ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11231-ACR|
|Human HIST1H3A ORF mammalian expression plasmid, N-GFPSpark tag||HG11231-ANG|
|Human HIST1H3A ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG11231-ANR|
|Human HIST1H3A ORF mammalian expression plasmid, C-Flag tag||HG11231-CF|
|Human HIST1H3A ORF mammalian expression plasmid, C-His tag||HG11231-CH|
|Human HIST1H3A ORF mammalian expression plasmid, C-Myc tag||HG11231-CM|
|Human HIST1H3A ORF mammalian expression plasmid, C-HA tag||HG11231-CY|
|Human HIST1H3A Gene cDNA clone plasmid||HG11231-M|
|Human HIST1H3A ORF mammalian expression plasmid, N-Flag tag||HG11231-NF|
|Human HIST1H3A ORF mammalian expression plasmid, N-His tag||HG11231-NH|
|Human HIST1H3A ORF mammalian expression plasmid, N-Myc tag||HG11231-NM|
|Human HIST1H3A ORF mammalian expression plasmid, N-HA tag||HG11231-NY|
|Human HIST1H3A natural ORF mammalian expression plasmid||HG11231-UT|
|Learn more about expression Vectors|
Histone H3.1, also known as HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, is a member of the histone H3 family which is a core component of nucleosome. It is expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.