|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human PDIA4 ORF mammalian expression plasmid, C-GFPSpark tag||HG13333-ACG|
|Human PDIA4 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13333-ACR|
|Human PDIA4 ORF mammalian expression plasmid, C-Flag tag||HG13333-CF|
|Human PDIA4 ORF mammalian expression plasmid, C-His tag||HG13333-CH|
|Human PDIA4 ORF mammalian expression plasmid, C-Myc tag||HG13333-CM|
|Human PDIA4 ORF mammalian expression plasmid, C-HA tag||HG13333-CY|
|Human PDIA4 Gene cDNA clone plasmid||HG13333-G|
|Human PDIA4 ORF mammalian expression plasmid, N-Flag tag||HG13333-NF|
|Human PDIA4 ORF mammalian expression plasmid, N-His tag||HG13333-NH|
|Human PDIA4 ORF mammalian expression plasmid, N-Myc tag||HG13333-NM|
|Human PDIA4 ORF mammalian expression plasmid, N-HA tag||HG13333-NY|
|Human PDIA4 natural ORF mammalian expression plasmid||HG13333-UT|
|Learn more about expression Vectors|
ERP72, also known as PDIA4, is an endoplasmic reticulum luminal protein which belongs to the protein disulfide isomerase family. ERP72 is a stress protein and participates in the catalysis of protein-S-S-bond rearrangement. Both of PDIA4 and PDIA3 function as proteases, protein disulfide isomerases, phospholipases or an arrangement of these. ERP72 compose part of a large chaperone multiprotein complex comprising CABP1, DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX.