|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human NTMT1 ORF mammalian expression plasmid, C-GFPSpark tag||HG11222-ACG|
|Human NTMT1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11222-ACR|
|Human NTMT1 ORF mammalian expression plasmid, N-GFPSpark tag||HG11222-ANG|
|Human NTMT1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG11222-ANR|
|Human NTMT1 ORF mammalian expression plasmid, C-Flag tag||HG11222-CF|
|Human NTMT1 ORF mammalian expression plasmid, C-His tag||HG11222-CH|
|Human NTMT1 ORF mammalian expression plasmid, C-Myc tag||HG11222-CM|
|Human NTMT1 ORF mammalian expression plasmid, C-HA tag||HG11222-CY|
|Human NTMT1 Gene cDNA clone plasmid||HG11222-M|
|Human NTMT1 ORF mammalian expression plasmid, N-Flag tag||HG11222-NF|
|Human NTMT1 ORF mammalian expression plasmid, N-His tag||HG11222-NH|
|Human NTMT1 ORF mammalian expression plasmid, N-Myc tag||HG11222-NM|
|Human NTMT1 ORF mammalian expression plasmid, N-HA tag||HG11222-NY|
|Human NTMT1 natural ORF mammalian expression plasmid||HG11222-UT|
|Learn more about expression Vectors|
Methyltransferase-like protein 11A, also known as METTL11A, is a member of the methyltransferase superfamily and METTL11 family. Methyltransferase is a type of transferase enzyme which transfers a methyl group from a donor to an acceptor. Methylation often occurs on nucleic bases in DNA or amino acids in protein structures. Methytransferase uses a reactive methyl group bound to sulfur in S-adenosyl methionine (SAM) as the methyl donor. DNA methylation is often utilized to silence and regulate genes without changing the original DNA sequence. This methylation occurs on cytosine residues. DNA methylation may be necessary for normal growth from embryonic stages in mammals. Methylation can serve to protect DNA from enzymatic cleavage, since restriction enzymes are unable to bind and recognize externally modified sequences. This is especially useful in bacterial restriction modification systems which use restriction enzymes to cleave foreign DNA while keeping their own DNA protected by methylation. Methylation of amino acids in the formation of proteins leads to more diversity of possible amino acids and therefore more diversity of function. The methylation reaction occurs on nitrogen atoms either on the N terminus or side-chain position of the protein and are usually irreversible.