|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human ICAM4 ORF mammalian expression plasmid, C-GFPSpark tag||HG13327-ACG|
|Human ICAM4 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13327-ACR|
|Human ICAM4 ORF mammalian expression plasmid, C-Flag tag||HG13327-CF|
|Human ICAM4 ORF mammalian expression plasmid, C-His tag||HG13327-CH|
|Human ICAM4 ORF mammalian expression plasmid, C-Myc tag||HG13327-CM|
|Human ICAM4 ORF mammalian expression plasmid, C-HA tag||HG13327-CY|
|Human ICAM4 Gene cDNA clone plasmid||HG13327-G|
|Human ICAM4 ORF mammalian expression plasmid, N-Flag tag||HG13327-NF|
|Human ICAM4 ORF mammalian expression plasmid, N-His tag||HG13327-NH|
|Human ICAM4 ORF mammalian expression plasmid, N-Myc tag||HG13327-NM|
|Human ICAM4 ORF mammalian expression plasmid, N-HA tag||HG13327-NY|
|Human ICAM4 natural ORF mammalian expression plasmid||HG13327-UT|
|Learn more about expression Vectors|
ICAM4, also known as CD242, is a member of the?immunoglobulin superfamily, ICAM family. ICAM4 contains 2?Ig-like C2-type (immunoglobulin-like) domains. It is similar to the intercellular adhesion molecule (ICAM) protein family. ICAM4 binds to the leukocyte adhesion LFA-1 protein. ICAM4's first reported receptors were CD11a/CD18 and CD11b/CD18. ICAM4 functions as a ligand for the monocyte/macrophage-specific CD11c/CD18. Deletion of the individual immunoglobulin domains of ICAM4 demonstrated that both its domains contain binding sites for CD11c/CD18. CD11c/CD18 is expressed on macrophages in spleen and bone marrow. Inhibition of erythrophagocytosis by anti-ICAM4 and anti-integrin antibodies suggests a role for these interactions in removal of senescent red cells.