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HSP27 / HSPB1 Antibody, Rabbit MAb

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HSPB2Antibody Product Information
Immunogen:Recombinant Human HSP27 protein (Catalog#10351-H08E)
Clone ID:155
Ig Type:Rabbit IgG
Concentration:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was obtained from a rabbit immunized with purified, recombinant Human HSP27 (rh HSP27; Catalog#10351-H08E; NP_001532.1; Met1-Lys205).
HSPB2Antibody Usage Guide
Specificity:Human HSP27
Application:ICC/IF, IF, FCM

ICC/IF: 10-25 μg/mL

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Images
HSP27 / HSPB1 Antibody, Rabbit MAb, Immunofluorescence
[Click to enlarge image]
Caption:
Immunofluorescence staining of Human HSP27 in A431 or SKBR3 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Rabbit anti-Human HSP27 monoclonal antibody (15 µg/ml). Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (left panel, captured by laser confocal scanning microscope; right panel, captured by fluorescence microscope), countstained with DAPI (blue). Positive staining was localized to cytoplasm.
HSPB1 Antibody, Rabbit MAb, Flow cytometric analysis
[Click to enlarge image]
Caption:
Flow cytometric analysis of Human HSP27 on HeLa cells. Cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human HSP27, then a FITC-conjugated second step antibody. The histogram were derived from events with the forward and side light-scatter characteristics of intact cells.
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Size / Price
Catalog:10351-R155-100
List Price: $290.00  (Save $0.00)
Price:$290.00      [How to order]
AvailabilityIn Stock
Images
  • HSP27 / HSPB1 Antibody, Rabbit MAb, Immunofluorescence
  • HSPB1 Antibody, Rabbit MAb, Flow cytometric analysis
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