|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human TFAP2C ORF mammalian expression plasmid, C-GFPSpark tag||HG13115-ACG|
|Human TFAP2C ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13115-ACR|
|Human TFAP2C ORF mammalian expression plasmid, N-GFPSpark tag||HG13115-ANG|
|Human TFAP2C ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG13115-ANR|
|Human TFAP2C ORF mammalian expression plasmid, C-Flag tag||HG13115-CF|
|Human TFAP2C ORF mammalian expression plasmid, C-His tag||HG13115-CH|
|Human TFAP2C ORF mammalian expression plasmid, C-Myc tag||HG13115-CM|
|Human TFAP2C ORF mammalian expression plasmid, C-HA tag||HG13115-CY|
|Human TFAP2C Gene cDNA clone plasmid||HG13115-G|
|Human TFAP2C ORF mammalian expression plasmid, N-Flag tag||HG13115-NF|
|Human TFAP2C ORF mammalian expression plasmid, N-His tag||HG13115-NH|
|Human TFAP2C ORF mammalian expression plasmid, N-Myc tag||HG13115-NM|
|Human TFAP2C ORF mammalian expression plasmid, N-HA tag||HG13115-NY|
|Human TFAP2C natural ORF mammalian expression plasmid||HG13115-UT|
|Learn more about expression Vectors|
TFAP2C, also known as AP2-GAMMA, is a member of the activating protein 2 family of transcription factors. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. TFAP2C may be prognostic indicators for patients with breast tumors. TFAP2C gene has been tested for association to diseases (Breast Neoplasms; Carcinoma) and proposed to participate in processes (cell-cell signaling, male gonad development, regulation of transcription from RNA polymerase II promoter). Proteins are expected to have molecular functions (DNA binding, protein binding, protein dimerization activity, transcription factor activity) and to localize in various compartments (membrane, nucleus).