|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human ABL1 ORF mammalian expression plasmid, C-GFPSpark tag||HG11199-ACG|
|Human ABL1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11199-ACR|
|Human ABL1 ORF mammalian expression plasmid, N-GFPSpark tag||HG11199-ANG|
|Human ABL1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG11199-ANR|
|Human ABL1 ORF mammalian expression plasmid, C-Flag tag||HG11199-CF|
|Human ABL1 ORF mammalian expression plasmid, C-His tag||HG11199-CH|
|Human ABL1 ORF mammalian expression plasmid, C-Myc tag||HG11199-CM|
|Human ABL1 ORF mammalian expression plasmid, C-HA tag||HG11199-CY|
|Human ABL1 Gene cDNA clone plasmid||HG11199-M|
|Human ABL1 ORF mammalian expression plasmid, N-Flag tag||HG11199-NF|
|Human ABL1 ORF mammalian expression plasmid, N-His tag||HG11199-NH|
|Human ABL1 ORF mammalian expression plasmid, N-Myc tag||HG11199-NM|
|Human ABL1 ORF mammalian expression plasmid, N-HA tag||HG11199-NY|
|Human ABL1 natural ORF mammalian expression plasmid||HG11199-UT|
|Learn more about expression Vectors|
c-Abl belongs to the class of tyrosine kinases and is the prototype of a subfamily which includes two members, c-Abl and Arg (Abl-related gene). Both proteins are localized at the cell membrane, actin cytoskeleton and cytosol, and c-Abl is present in the nucleus as well. c-Abl is a non-receptor tyrosine kinase that participates in multiple signaling pathways linking the cell surface, cytoskeleton, and the nucleus. Recent in vitro studies have also linked c-Abl to amyloid-beta-induced toxicity and tau phosphorylation. c-Abl has been implicated in many cellular processes including differentiation, division, adhesion, death, and stress response. c-Abl is a latent tyrosine kinase that becomes activated in response to numerous extra- and intra-cellular stimuli. The c-Abl protein is a ubiquitously expressed nonreceptor tyrosine kinase involved in the development and function of many mammalian organ systems, including the immune system and bone. It regulates the cellular response to TAM through functional interaction with the estrogen receptor, which suggests c-Abl as a therapeutic target and a prognostic tumor marker for breast cancer. c-Abl also plays a key role in signaling chemokine-induced T-cell migration. In addition, c-Abl contains NLSs (nuclear localization signals) and DNA-binding sequences important for nuclear functions. c-Abl has become an important therapeutic target in human chronic myeloid leukaemia.