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CCNE1 / Cyclin-E1 Antibody, Mouse MAb

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Human CCNE1 Antibody Product Information
Immunogen:Recombinant Human CCNE1 / Cyclin-E1 protein (Catalog#10902-H07B)
Clone ID:03
Ig Type:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CCNE1 / Cyclin-E1 (rh CCNE1 / Cyclin-E1; Catalog#10902-H07B; NP_001229.1; Met1-Ala410). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Human CCNE1 Antibody Usage Guide
Specificity:Human CCNE1 / Cyclin-E1
Application:ICC/IF, IF

ICC/IF: 10-25 μg/mL

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Human CCNE1 Antibody IHC Application Image
[Click to enlarge image]
Immunofluorescence staining of Human CCNE1 in MCF7 or Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-Human CCNE1 monoclonal antibody (15 µg/ml). Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (left panel, captured by laser confocal scanning microscope; right panel, captured by fluorescence microscope), countstained with Alexa Fluor® 546-conjugated phallotoxins (red) and DAPI (blue). Positive staining was localized to nucleus.
CCNE1 / Cyclin-E1 Antibody, Mouse MAb, Immunofluorescence
Other CCNE1 Antibody Products
Cyclin E1/CCNE1 Background

Cyclin E1 is a member of the highly conserved cyclin family and belongs to the E-type cyclin that functions as a regulator of S phase entry and progression in mammalian cells. Cyclin E1 serves as regulatory subunits that bind, activate, and provide substrate for its associated cyclin-dependent kinase2 (CDK2), whose activity is essential for cell cycle G1 / S transition. Over expression of this encoding gene has been found in many tumors, which results in chromosome instability and by extension, induce tumorigenesis. This protein was also found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. In general, cyclin E1, as an activator of phospho-CDK2 (pCDK2), is important for cell cycle progression and is frequently overexpressed in cancer cells.

Human Cyclin E1/CCNE1 References
  • Honda R, et al. (2005) The structure of cyclin E1 / CDK2: implications for CDK2 activation and CDK2-independent roles. The EMBO Journal. 24: 452-63.
  • Geng Y, et al. (2007) Kinase-Independent Function of Cyclin E. 25(1): 127-39.
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