|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human IGFBP7 ORF mammalian expression plasmid, C-GFPSpark tag||HG13100-ACG|
|Human IGFBP7 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13100-ACR|
|Human IGFBP7 ORF mammalian expression plasmid, C-Flag tag||HG13100-CF|
|Human IGFBP7 ORF mammalian expression plasmid, C-His tag||HG13100-CH|
|Human IGFBP7 ORF mammalian expression plasmid, C-Myc tag||HG13100-CM|
|Human IGFBP7 ORF mammalian expression plasmid, C-HA tag||HG13100-CY|
|Human IGFBP7 Gene cDNA clone plasmid||HG13100-G|
|Human IGFBP7 ORF mammalian expression plasmid, N-Flag tag||HG13100-NF|
|Human IGFBP7 ORF mammalian expression plasmid, N-His tag||HG13100-NH|
|Human IGFBP7 ORF mammalian expression plasmid, N-Myc tag||HG13100-NM|
|Human IGFBP7 ORF mammalian expression plasmid, N-HA tag||HG13100-NY|
|Human IGFBP7 natural ORF mammalian expression plasmid||HG13100-UT|
|Learn more about expression Vectors|
Insulin like growth factor binding protein 7 (IGFBP7) is a member of the IGFBP family. It has been identified in colorectal adenocarcinoma (CRC) cell lines. The Insulin-like growth factor-binding protein also known as IGFBP serves as a carrier protein for Insulin-like growth factor 1. IGFBPs are clearly distinct but are sharing regions with strong homology. All members of the IGFBP family bind IGF-I and IGF-II with about equal affinity. Insulin-like growth factor (IGF) binding proteins (IGFBPs) have been shown to either inhibit or enhance the action of IGF, or act in an IGF-independent manner in the prostate. IGFBP7 could inhibit cell growth, decrease soft agar colony formation activity and induce apoptosis in RKO and SW620 cells. There is mounting evidence that the structure of the IGFBP proteins plays a key role in the regulation of IGF bioavailability, by modulating its molecular size, capillary membrane permeability, target tissue specificity, cell membrane adherence and IGF affinity.