|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human TMED1 ORF mammalian expression plasmid, C-GFPSpark tag||HG13302-ACG|
|Human TMED1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13302-ACR|
|Human TMED1 ORF mammalian expression plasmid, C-Flag tag||HG13302-CF|
|Human TMED1 ORF mammalian expression plasmid, C-His tag||HG13302-CH|
|Human TMED1 ORF mammalian expression plasmid, C-Myc tag||HG13302-CM|
|Human TMED1 ORF mammalian expression plasmid, C-HA tag||HG13302-CY|
|Human TMED1 Gene cDNA clone plasmid||HG13302-G|
|Human TMED1 ORF mammalian expression plasmid, N-Flag tag||HG13302-NF|
|Human TMED1 ORF mammalian expression plasmid, N-His tag||HG13302-NH|
|Human TMED1 ORF mammalian expression plasmid, N-Myc tag||HG13302-NM|
|Human TMED1 ORF mammalian expression plasmid, N-HA tag||HG13302-NY|
|Human TMED1 natural ORF mammalian expression plasmid||HG13302-UT|
|Learn more about expression Vectors|
TMED1 belongs to the EMP24/GP25L family. It contains 1 GOLD domain and is widely expressed. TMED1 binds to its receptor IL1RL1 and results in the activation of DNA binding by nuclear factor NF-kappa-B or transcription from the IL8 promoter and most likely requires other proteins to elicit these activities. Dendritic cells from Peyer's patches (but not from spleen) express TMED1 in response to treatment with LPS. TMED1 may play a role in vesicular protein trafficking, mainly in the early secretory pathway. It may act as a cargo receptor at the lumenal side for incorporation of secretory cargo molecules into transport vesicles and may be involved in vesicle coat formation at the cytoplasmic side.