|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human MTSS1 ORF mammalian expression plasmid, C-GFPSpark tag||HG13085-ACG|
|Human MTSS1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13085-ACR|
|Human MTSS1 ORF mammalian expression plasmid, N-GFPSpark tag||HG13085-ANG|
|Human MTSS1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG13085-ANR|
|Human MTSS1 ORF mammalian expression plasmid, C-Flag tag||HG13085-CF|
|Human MTSS1 ORF mammalian expression plasmid, C-His tag||HG13085-CH|
|Human MTSS1 ORF mammalian expression plasmid, C-Myc tag||HG13085-CM|
|Human MTSS1 ORF mammalian expression plasmid, C-HA tag||HG13085-CY|
|Human MTSS1 Gene cDNA clone plasmid||HG13085-G|
|Human MTSS1 ORF mammalian expression plasmid, N-Flag tag||HG13085-NF|
|Human MTSS1 ORF mammalian expression plasmid, N-His tag||HG13085-NH|
|Human MTSS1 ORF mammalian expression plasmid, N-Myc tag||HG13085-NM|
|Human MTSS1 ORF mammalian expression plasmid, N-HA tag||HG13085-NY|
|Human MTSS1 natural ORF mammalian expression plasmid||HG13085-UT|
|Learn more about expression Vectors|
MTSS1 (Metastasis suppressor 1), also known as Missing in metastasis (MIM), is a tissue-specific regulator of plasma membrane dynamics. MTSS1 is well described for its function as a metastasis suppressor gene and is expressed in a variety of tissues. MTSS1 might be involved in shaping neuronal membranes in vivo. MTSS1 deforms phosphoinositide-rich membranes through its I-BAR domain and interacts with actin monomers through its WH2 domain. MTSS1/MIM was first identified as a metastasis suppressor missing in metastatic bladder carcinoma cell lines. MTSS1 is a prognostic indicator of disease-free survival in breast cancer patients and demonstrates the ability to play a role in governing the metastatic nature of breast cancer cells. MTSS1 may serve as a useful biomarker for the prediction of outcome of gastric cancer. The down-regulation of MTSS1 that may be caused by DNA methylation was also observed in many other types of cancer.Recent work proposed that MIM also potentiates Sonic hedgehog (Shh)-induced gene expression. MTSS1 as a multiple functional molecular player and has an important role in development, carcinogenesis and metastasis.