|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human CALCB ORF mammalian expression plasmid, C-GFPSpark tag||HG13283-ACG|
|Human CALCB ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13283-ACR|
|Human CALCB ORF mammalian expression plasmid, C-Flag tag||HG13283-CF|
|Human CALCB ORF mammalian expression plasmid, C-His tag||HG13283-CH|
|Human CALCB ORF mammalian expression plasmid, C-Myc tag||HG13283-CM|
|Human CALCB ORF mammalian expression plasmid, C-HA tag||HG13283-CY|
|Human CALCB Gene cDNA clone plasmid||HG13283-G|
|Human CALCB ORF mammalian expression plasmid, N-Flag tag||HG13283-NF|
|Human CALCB ORF mammalian expression plasmid, N-His tag||HG13283-NH|
|Human CALCB ORF mammalian expression plasmid, N-Myc tag||HG13283-NM|
|Human CALCB ORF mammalian expression plasmid, N-HA tag||HG13283-NY|
|Human CALCB natural ORF mammalian expression plasmid||HG13283-UT|
|Learn more about expression Vectors|
CALCB, also known as CGPR and calcitonin 2, belongs to the calcitonin family. CALCB is a calcitonin (CT) peptide which may play a role in the mediation of human inflammatory diseases. It is highly expressed in the skin, blood, and cerebrospinal fluid. CGRP immunolabeling (IL) was detected in epidermal keratinocytes at levels that were especially high and widespread in the skin of humans from locations afflicted with postherpetic neuralgia (PHN) and complex region pain syndrome type 1 (CRPS), of monkeys infected with simian immunodeficiency virus, and of rats subjected to L5/L6 spinal nerve ligation, sciatic nerve chronic constriction, and subcutaneous injection of complete Freund's adjuvant. Increased CGRP-IL was also detected in epidermal keratinocytes of transgenic mice with keratin-14 promoter driven overexpression of noggin, an antagonist to BMP-4 signaling. CGPR dilates a variety of vessels including the coronary, cerebral and systemic vasculature.