|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human GOLM1 ORF mammalian expression plasmid, C-GFPSpark tag||HG13066-ACG|
|Human GOLM1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13066-ACR|
|Human GOLM1 ORF mammalian expression plasmid, C-Flag tag||HG13066-CF|
|Human GOLM1 ORF mammalian expression plasmid, C-His tag||HG13066-CH|
|Human GOLM1 ORF mammalian expression plasmid, C-Myc tag||HG13066-CM|
|Human GOLM1 ORF mammalian expression plasmid, C-HA tag||HG13066-CY|
|Human GOLM1 Gene cDNA clone plasmid||HG13066-G|
|Human GOLM1 ORF mammalian expression plasmid, His tag||HG13066-G-H|
|Human GOLM1 ORF mammalian expression plasmid, N-Flag tag||HG13066-NF|
|Human GOLM1 ORF mammalian expression plasmid, N-His tag||HG13066-NH|
|Human GOLM1 ORF mammalian expression plasmid, N-Myc tag||HG13066-NM|
|Human GOLM1 ORF mammalian expression plasmid, N-HA tag||HG13066-NY|
|Human GOLM1 natural ORF mammalian expression plasmid||HG13066-UT|
|Learn more about expression Vectors|
Golgi membrane protein 1, also known as Golgi membrane protein GP73, Golgi phosphoprotein 2 and GOLM1, is a protein which belongs to the GOLM1 / CASC4 family. GOLM1 is widely expressed. It is highly expressed in colon, prostate, trachea and stomach. It is expressed at lower level in testis, muscle, lymphoid tissues, white blood cells and spleen. It is predominantly expressed by cells of the epithelial lineage. GOLM1 is expressed at low level in normal liver. Expression significantly increases in virus (HBV, HCV) infected liver. Expression of GOLM1 does not increase in liver disease due to non-viral causes (alcohol-induced liver disease, autoimmune hepatitis). Increased expression in hepatocytes appears to be a general feature of advanced liver disease. In liver tissue from patients with adult giant-cell hepatitis (GCH), GOLM1 is strongly expressed in hepatocyte-derived syncitial giant cells. GOLM1 is constitutively expressed by biliary epithelial cells but not by hepatocytes.