|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human CCDC47 ORF mammalian expression plasmid, C-GFPSpark tag||HG13274-ACG|
|Human CCDC47 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13274-ACR|
|Human CCDC47 ORF mammalian expression plasmid, C-Flag tag||HG13274-CF|
|Human CCDC47 ORF mammalian expression plasmid, C-His tag||HG13274-CH|
|Human CCDC47 ORF mammalian expression plasmid, C-Myc tag||HG13274-CM|
|Human CCDC47 ORF mammalian expression plasmid, C-HA tag||HG13274-CY|
|Human CCDC47 Gene cDNA clone plasmid||HG13274-G|
|Human CCDC47 ORF mammalian expression plasmid, N-Flag tag||HG13274-NF|
|Human CCDC47 ORF mammalian expression plasmid, N-His tag||HG13274-NH|
|Human CCDC47 ORF mammalian expression plasmid, N-Myc tag||HG13274-NM|
|Human CCDC47 ORF mammalian expression plasmid, N-HA tag||HG13274-NY|
|Human CCDC47 natural ORF mammalian expression plasmid||HG13274-UT|
|Learn more about expression Vectors|
CCDC47 gene is expressed at high level. The gene contains 16 distinct gt-ag introns. Transcription produces 9 different mRNAs, 6 alternatively spliced variants and 3 unspliced forms. There are 3 probable alternative promotors, 3 non overlapping alternative last exons and 8 validated alternative polyadenylation sites. The mRNAs appear to differ by truncation of the 5' end, truncation of the 3' end, presence or absence of a cassette exon, overlapping exons with different boundaries. Functionally, CCDC47 gene has been proposed to participate in processes such as calcium ion homeostasis, embryo development, ER overload response and post-embryonic development. CCDC47 are expected to have molecular function (calcium ion binding) and to localize in various compartments (membrane, endoplasmic reticulum, integral to membrane, microsome).