|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human CANT1 ORF mammalian expression plasmid, C-GFPSpark tag||HG13266-ACG|
|Human CANT1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13266-ACR|
|Human CANT1 ORF mammalian expression plasmid, C-Flag tag||HG13266-CF|
|Human CANT1 ORF mammalian expression plasmid, C-His tag||HG13266-CH|
|Human CANT1 ORF mammalian expression plasmid, C-Myc tag||HG13266-CM|
|Human CANT1 ORF mammalian expression plasmid, C-HA tag||HG13266-CY|
|Human CANT1 Gene cDNA clone plasmid||HG13266-G|
|Human CANT1 ORF mammalian expression plasmid, N-Flag tag||HG13266-NF|
|Human CANT1 ORF mammalian expression plasmid, N-His tag||HG13266-NH|
|Human CANT1 ORF mammalian expression plasmid, N-Myc tag||HG13266-NM|
|Human CANT1 ORF mammalian expression plasmid, N-HA tag||HG13266-NY|
|Human OLFML3 natural ORF mammalian expression plasmid||HG13266-UT|
|Learn more about expression Vectors|
CANT1(calcium activated nucleotidase 1) belongs to the apyrase family. Apyrase is a calcium-activated plasma membrane-bound enzyme (magnesium can also activate it) (EC 126.96.36.199) that catalyses the hydrolysis of ATP to yield AMP and inorganic phosphate. Two isoenzymes are found in commercial preparations from S. tuberosum. One with a higher ratio of substrate selectivity for ATP: ADP and another with no selectivity. It can also act on ADP and other nucleoside triphosphates and diphosphates with the general reaction being NTP -> NDP + Pi -> NMP + 2Pi. The salivary apyrases of blood-feeding arthropods are nucleotide hydrolysing enzymes are implicated in the inhibition of host platelet aggregation through the hydrolysis of extracellular adenosine diphosphate. CANT1 functions as a calcium-dependent nucleotidase with a preference for UDP. Defects in CANT1 are the cause of desbuquois dysplasia.