|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human CDCP1 ORF mammalian expression plasmid, C-GFPSpark tag||HG13262-ACG|
|Human CDCP1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13262-ACR|
|Human CDCP1 ORF mammalian expression plasmid, C-Flag tag||HG13262-CF|
|Human CDCP1 ORF mammalian expression plasmid, C-His tag||HG13262-CH|
|Human CDCP1 ORF mammalian expression plasmid, C-Myc tag||HG13262-CM|
|Human CDCP1 ORF mammalian expression plasmid, C-HA tag||HG13262-CY|
|Human CDCP1 Gene cDNA clone plasmid||HG13262-G|
|Human CDCP1 ORF mammalian expression plasmid, N-Flag tag||HG13262-NF|
|Human CDCP1 ORF mammalian expression plasmid, N-His tag||HG13262-NH|
|Human CDCP1 ORF mammalian expression plasmid, N-Myc tag||HG13262-NM|
|Human CDCP1 ORF mammalian expression plasmid, N-HA tag||HG13262-NY|
|Human CDCP1 natural ORF mammalian expression plasmid||HG13262-UT|
|Learn more about expression Vectors|
CDCP1 contains three extracellular CUB domains. It is a putative stem cell marker that is highly expressed in some human cancer cells and in both, typical and atypical (cancerous) colons. It interacts with CDH2/N-cadherin, CDH3/P-cadherin, SDC1/syndecan-1, SDC4/syndecan-4 and the serine protease ST14/MT-SP1. It also interacts with SRC and PRKCG/protein kinase C gamma. CDCP1 is taken as a key regulator of EGF/EGFR-induced cell migration. It has been shown that signaling via EGF/EGFR induces migration of ovarian cancer Caov3 and OVCA420 cells with concomitant up-regulation of CDCP1 mRNA and protein. Consistent with a role in cell migration CDCP1 relocates from cell-cell junctions to punctate structures on filopodia after activation of EGFR. It may be involved in cell adhesion and cell matrix association. It also may play a role in the regulation of anchorage versus migration or proliferation versus differentiation via its phosphorylation. It has been taken as a novel marker for leukemia diagnosis and for immature hematopoietic stem cell subsets.