|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human S100A8 ORF mammalian expression plasmid, C-GFPSpark tag||HG11138-ACG|
|Human S100A8 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11138-ACR|
|Human S100A8 ORF mammalian expression plasmid, C-Flag tag||HG11138-CF|
|Human S100A8 ORF mammalian expression plasmid, C-His tag||HG11138-CH|
|Human S100A8 ORF mammalian expression plasmid, C-Myc tag||HG11138-CM|
|Human S100A8 ORF mammalian expression plasmid, C-HA tag||HG11138-CY|
|Human S100A8 Gene cDNA clone plasmid||HG11138-M|
|Human S100A8 ORF mammalian expression plasmid, N-Flag tag||HG11138-NF|
|Human S100A8 ORF mammalian expression plasmid, N-His tag||HG11138-NH|
|Human S100A8 ORF mammalian expression plasmid, N-Myc tag||HG11138-NM|
|Human S100A8 ORF mammalian expression plasmid, N-HA tag||HG11138-NY|
|Human S100A8 natural ORF mammalian expression plasmid||HG11138-UT|
|Learn more about expression Vectors|
S100A8 is a member of the S100 protein family containing 2EF-hand calcium-binding motifs. S100 proteins are involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. Altered expression of S100A8 protein is associated with various diseases and cancers. S100A8 may have an immunoregulatory role by contributing to the regulation of fetal-maternal interactions. It may play a protective role and its absence may allow infiltration by maternal cells, a process eventually manifesting as resorption. The heterodimeric S100 protein complex S100A8/A9 which has been shown to be involved in inflammatory and neoplastic disorders. The complex can induce cell proliferation, or apoptosis, inflammation, collagen synthesis, and cell migration. S100A8/A9 has emerged as important pro-inflammatory mediator in acute and chronic inflammation. More recently, increased S100A8 and S100A9 levels were also detected in various human cancers, presenting abundant expression in neoplastic tumor cells as well as infiltrating immune cells. On the one hand, S100A8/A9 is a powerful apoptotic agent produced by immune cells, making it a very fascinating tool in the battle against cancer. It spears the risk to induce auto-immune response and may serve as a lead compound for cancer-selective therapeutics. In contrast, S100A8/A9 expression in cancer cells has also been associated with tumor development, cancer invasion or metastasis. Altogether, its expression and potential cytokine-like function in inflammation and in cancer suggests that S100A8/A9 may play a key role in inflammation-associated cancer.