|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human CST9L ORF mammalian expression plasmid, C-GFPSpark tag||HG13243-ACG|
|Human CST9L ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13243-ACR|
|Human CST9L ORF mammalian expression plasmid, C-Flag tag||HG13243-CF|
|Human CST9L ORF mammalian expression plasmid, C-His tag||HG13243-CH|
|Human CST9L ORF mammalian expression plasmid, C-Myc tag||HG13243-CM|
|Human CST9L ORF mammalian expression plasmid, C-HA tag||HG13243-CY|
|Human CST9L Gene cDNA clone plasmid||HG13243-G|
|Human CST9L ORF mammalian expression plasmid, N-Flag tag||HG13243-NF|
|Human CST9L ORF mammalian expression plasmid, N-His tag||HG13243-NH|
|Human CST9L ORF mammalian expression plasmid, N-Myc tag||HG13243-NM|
|Human CST9L ORF mammalian expression plasmid, N-HA tag||HG13243-NY|
|Human CST9L natural ORF mammalian expression plasmid||HG13243-UT|
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Testatin is a member of the Cystatin family. Cystatins comprise genes that all show expression patterns that are strikingly restricted to reproductive tissue. Cystatins are a family of cysteine protease inhibitors with homology to chicken cystatin. There are typically about 115 amino acids in this family. They are largely acidic, contain four conserved cysteine residues known to form two disulfide bonds, may be glycosylated and/or phosphorylated, with similarity to fetuins, kininogens, stefins, histidine-rich glycoproteins and cystatin-related proteins. Testatin shows homology to family 2 cystatins, a group of broadly expressed small secretory proteins that are inhibitors of cysteine proteases in vitro but whose in vivo functions are unclear. It is expressed in germ cells and somatic cells in reproductive tissues. Testatin is considered a strong candidate for involvement in early testis development. Testatin expression is maintained in the adult Sertoli cell, and it can also be found in a small population of germ cells.