|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human AZGP1 ORF mammalian expression plasmid, C-GFPSpark tag||HG13242-ACG|
|Human AZGP1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13242-ACR|
|Human AZGP1 ORF mammalian expression plasmid, C-Flag tag||HG13242-CF|
|Human AZGP1 ORF mammalian expression plasmid, C-His tag||HG13242-CH|
|Human AZGP1 ORF mammalian expression plasmid, C-Myc tag||HG13242-CM|
|Human AZGP1 ORF mammalian expression plasmid, C-HA tag||HG13242-CY|
|Human AZGP1 Gene cDNA clone plasmid||HG13242-G|
|Human AZGP1 ORF mammalian expression plasmid, N-Flag tag||HG13242-NF|
|Human AZGP1 ORF mammalian expression plasmid, N-His tag||HG13242-NH|
|Human AZGP1 ORF mammalian expression plasmid, N-Myc tag||HG13242-NM|
|Human AZGP1 ORF mammalian expression plasmid, N-HA tag||HG13242-NY|
|Human AZGP1 natural ORF mammalian expression plasmid||HG13242-UT|
|Learn more about expression Vectors|
Alpha-2-glycoprotein, also known as AZGP1, belongs to the MHC class I family. It can be detected in body fluids such as serum, sweat, and seminal and breast cyst fluids. It has been shown that alpha-2-glycoprotein can stimulate lipolysis by adipocytes in vivo and in vitro. Thus it is believed that alpha-2-glycoprotein plays an important role in the regulation of body weight, and age-dependent changes in genetically influenced obesity, and it also regulates melanin production by normal and malignant melanocytes. Alpha-2-glycoprotein is produced by both white and brown fat adipocytes and may act in a local autocrine fashion in the reduction of adiposity in cachexia.