|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human CES3 ORF mammalian expression plasmid, C-GFPSpark tag||HG11129-ACG|
|Human CES3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11129-ACR|
|Human CES3 ORF mammalian expression plasmid, C-Flag tag||HG11129-CF|
|Human CES3 ORF mammalian expression plasmid, C-His tag||HG11129-CH|
|Human CES3 ORF mammalian expression plasmid, C-Myc tag||HG11129-CM|
|Human CES3 ORF mammalian expression plasmid, C-HA tag||HG11129-CY|
|Human CES3 Gene cDNA clone plasmid||HG11129-M|
|Human CES3 ORF mammalian expression plasmid, Flag tag||HG11129-M-F|
|Human CES3 ORF mammalian expression plasmid, N-Flag tag||HG11129-NF|
|Human CES3 ORF mammalian expression plasmid, N-His tag||HG11129-NH|
|Human CES3 ORF mammalian expression plasmid, N-Myc tag||HG11129-NM|
|Human CES3 ORF mammalian expression plasmid, N-HA tag||HG11129-NY|
|Human CES3 natural ORF mammalian expression plasmid||HG11129-UT|
|Learn more about expression Vectors|
Carboxylesterases hydrolyze esters of short-chain fatty acids and have roles in animals ranging from signal transduction to xenobiotic detoxification. In enzymology, a carboxylesterase is an enzyme that catalyzes the chemical reaction: a carboxylic ester + H2O = an alcohol + a carboxylate. Most enzymes from this group belong to the superfamily of hydrolases with alpha/beta protein fold (so called Alpha/beta hydrolase fold), specifically those acting on carboxylic ester bonds. The carboxylesterase family of evolutionarily related proteins (those with clear sequence homology to each other) includes a number of proteins with different substrate specificities, such as acetylcholinesterases. Carboxylesterase 3, also known as Liver carboxylesterase 31 homolog and CES3, is a endoplasmic reticulum lumen which belongs to the type-B carboxylesterase/lipase family. CES3 is involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. CES3 shows low catalytic efficiency for hydrolysis of CPT-11, a prodrug for camptothecin used in cancer therapeutics. CES3 is expressed in liver, colon and small intestine.