|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human AACS ORF mammalian expression plasmid, C-GFPSpark tag||HG11117-ACG|
|Human AACS ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11117-ACR|
|Human AACS ORF mammalian expression plasmid, N-GFPSpark tag||HG11117-ANG|
|Human AACS ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG11117-ANR|
|Human AACS ORF mammalian expression plasmid, C-Flag tag||HG11117-CF|
|Human AACS ORF mammalian expression plasmid, C-His tag||HG11117-CH|
|Human AACS ORF mammalian expression plasmid, C-Myc tag||HG11117-CM|
|Human AACS ORF mammalian expression plasmid, C-HA tag||HG11117-CY|
|人 AACS 基因全长ORF克隆||HG11117-M|
|Human AACS ORF mammalian expression plasmid, N-Flag tag||HG11117-NF|
|Human AACS ORF mammalian expression plasmid, N-His tag||HG11117-NH|
|Human AACS ORF mammalian expression plasmid, N-Myc tag||HG11117-NM|
|Human AACS ORF mammalian expression plasmid, N-HA tag||HG11117-NY|
|Human AACS natural ORF mammalian expression plasmid||HG11117-UT|
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Acetoacetyl-CoA Synthetase (AACS) is a novel cytosolic ketone body (acetoacetate)-specific ligase. The AACS in adipose tissue plays an important role in utilizing ketone body for the fatty acid-synthesis during adipose tissue development. It had been improved that Acetoacetyl-CoA Synthetase is an essential enzyme for the synthesis of fatty acid and cholesterol from ketone bodies, was found to be highly expressed in mouse adipose tissue, and GC box and C/EBPs motif were crucial for AACS promoter activity in 3T3-L1 adipocytes. Moreover, AACS promoter activity was controlled mainly by C/EBPalpha during adipogenesis. AACS gene expression is particularly abundant in white adipose tissue, as it is induced during adipocyte differentiation. The human AACS promoter is a PPARgamma target gene and that this nuclear receptor is recruited to the AACS promoter by direct interaction with Sp1 (stimulating protein-1). The Acetoacetyl-CoA Synthetase has important roles in the regulation of ketone body utilization in rat liver and that these hypocholesterolemic agents have the ability to remedy the impaired utilization of ketone bodies under the diabetic condition.