|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human MICA ORF mammalian expression plasmid, C-GFPSpark tag||HG12302-ACG|
|Human MICA ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG12302-ACR|
|Human MICA ORF mammalian expression plasmid, C-Flag tag||HG12302-CF|
|Human MICA ORF mammalian expression plasmid, C-His tag||HG12302-CH|
|Human MICA ORF mammalian expression plasmid, C-Myc tag||HG12302-CM|
|Human MICA ORF mammalian expression plasmid, C-HA tag||HG12302-CY|
|Human MICA Gene cDNA clone plasmid||HG12302-G|
|Human MICA ORF mammalian expression plasmid, N-Flag tag||HG12302-NF|
|Human MICA ORF mammalian expression plasmid, N-His tag||HG12302-NH|
|Human MICA ORF mammalian expression plasmid, N-Myc tag||HG12302-NM|
|Human MICA ORF mammalian expression plasmid, N-HA tag||HG12302-NY|
|Human MICA natural ORF mammalian expression plasmid||HG12302-UT|
|Learn more about expression Vectors|
MHC class I chain-related molecules A (MICA) is one of the genes in the HLA class I region, which belongs to MHC class I family. It is the member of the non-classical class I family that displays the greatest degree of polymorphism. The MICA protein product is expressed on the cell surface, although unlike canonical class I molecules does not seem to associate with beta-2-microglobulin. It is thought that MICA functions as a stress-induced antigen that is broadly recognized by NK cells, NKT cells, and most of the subtypes of T cells. The Natural killer group 2D (NKG2D), a C-type lectin-like activating immunoreceptor, is a receptor of MICA, which was detected on most gammadelta T cells, CD8+ alphabeta T cells, and natural killer (NK) cells. Effector cells from all these subsets could be stimulated by ligation of NKG2D. Engagement of NKG2D activated cytolytic responses of gammadelta T cells and NK cells against transfectants and epithelial tumor cells expressing MICA. The MICA system is a novel, avidin-free immunohistochemical detection system that provides a significant increase in sensitivity compared to traditional immunodetection systems.