|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human FDPS ORF mammalian expression plasmid, C-GFPSpark tag||HG13229-ACG|
|Human FDPS ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13229-ACR|
|Human FDPS ORF mammalian expression plasmid, N-GFPSpark tag||HG13229-ANG|
|Human FDPS ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG13229-ANR|
|Human FDPS ORF mammalian expression plasmid, C-Flag tag||HG13229-CF|
|Human FDPS ORF mammalian expression plasmid, C-His tag||HG13229-CH|
|Human FDPS ORF mammalian expression plasmid, C-Myc tag||HG13229-CM|
|Human FDPS ORF mammalian expression plasmid, C-HA tag||HG13229-CY|
|Human FDPS Gene cDNA clone plasmid||HG13229-G|
|Human FDPS ORF mammalian expression plasmid, N-Flag tag||HG13229-NF|
|Human FDPS ORF mammalian expression plasmid, N-His tag||HG13229-NH|
|Human FDPS ORF mammalian expression plasmid, N-Myc tag||HG13229-NM|
|Human FDPS ORF mammalian expression plasmid, N-HA tag||HG13229-NY|
|Human FDPS natural ORF mammalian expression plasmid||HG13229-UT|
|Learn more about expression Vectors|
Z-farnesyl diphosphate synthase (FDPS) is an enzyme belonging to the family of transferases, specifically those transferring aryl or alkyl groups other than methyl groups. Z-farnesyl diphosphate synthase (FDPS) functions as key enzyme in isoprenoid biosynthesis which catalyzes the formation of farnesyl diphosphate, a precurcor for several classes of essential metabolites. FDPS catalyzes the production of geranyl pyrophosphate and farnesyl pyrophosphate from isopentenyl pyrophosphate and dimethylallyl pyrophosphate. The resulting product, farnesyl pyrophosphate, is a key intermediate in cholesterol and sterol biosynthesis, a substrate for protein farnesylation and geranylgeranylation, and a ligand or agonist for certain hormone receptors and growth receptors. Drugs that inhibit this enzyme prevent the post-translational modifications of small GTPases and have been used to treat diseases related to bone resorption. Functions of FDPS may be inactivated by interferon-induced RSAD2. This inactivation may result of disruption of lipid rafts at the plasma membrane, and thus have an antiviral effect since many enveloped viruses need lipid rafts to bud efficiently out of the cell.