|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human ACBD6 ORF mammalian expression plasmid, C-GFPSpark tag||HG11112-ACG|
|Human ACBD6 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11112-ACR|
|Human ACBD6 ORF mammalian expression plasmid, N-GFPSpark tag||HG11112-ANG|
|Human ACBD6 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG11112-ANR|
|Human ACBD6 ORF mammalian expression plasmid, C-Flag tag||HG11112-CF|
|Human ACBD6 ORF mammalian expression plasmid, C-His tag||HG11112-CH|
|Human ACBD6 ORF mammalian expression plasmid, C-Myc tag||HG11112-CM|
|Human ACBD6 ORF mammalian expression plasmid, C-HA tag||HG11112-CY|
|Human ACBD6 Gene cDNA clone plasmid||HG11112-M|
|Human ACBD6 ORF mammalian expression plasmid, Flag tag||HG11112-M-F|
|Human ACBD6 ORF mammalian expression plasmid, N-Flag tag||HG11112-NF|
|Human ACBD6 ORF mammalian expression plasmid, N-His tag||HG11112-NH|
|Human ACBD6 ORF mammalian expression plasmid, N-Myc tag||HG11112-NM|
|Human ACBD6 ORF mammalian expression plasmid, N-HA tag||HG11112-NY|
|Human ACBD6 natural ORF mammalian expression plasmid||HG11112-UT|
|Learn more about expression Vectors|
Human acyl-coenzyme A binding domain-containing member 6 (ACBD6) is a modular protein that carries an acyl-CoA binding domain at its N terminus and two ankyrin motifs at its C terminus. In mammals, there are six members of the acyl-CoA binding domain-containing (ACBD) family, and their annotation is not uniform. All six ACBD proteins contain an ACB domain at the N terminus, but they do not share significant homology at the C-terminal region. ACBD6 is a 32 kDa protein that is predicted by sequence analysis to carry an ACB domain between residues 42 and 125 and two ANK motifs at its C terminus. This protein binds long-chain acyl-CoAs with a strong preference for unsaturated, C18:1-CoA and C20:4-CoA, over saturated, C16:0-CoA, acyl species. ACBD6 is not a ubiquitous protein, but it is expressed in hematopoietic tissues and appears to be restricted to primitive stem cells present in those tissues with functions in blood and vessel development. ACBD6 was detected in bone marrow, spleen, placenta, cord blood, circulating CD34+ progenitors, and embryonic-like stem cells derived from placenta. In placenta, the protein was only detected in CD34+ progenitor cells present in blood and in CD31+ endothelial cells surrounding the blood vessels. These cells were also positive for the marker CD133, and they probably constitute hemangiogenic stem cells, precursors of both blood and vessels. We propose that human ACBD6 represents a cellular marker for primitive progenitor cells with functions in hematopoiesis and vascular endothelium development.