|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human SLAMF8 ORF mammalian expression plasmid, C-GFPSpark tag||HG11101-ACG|
|Human SLAMF8 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11101-ACR|
|Human SLAMF8 ORF mammalian expression plasmid, C-Flag tag||HG11101-CF|
|Human SLAMF8 ORF mammalian expression plasmid, C-His tag||HG11101-CH|
|Human SLAMF8 ORF mammalian expression plasmid, C-Myc tag||HG11101-CM|
|Human SLAMF8 ORF mammalian expression plasmid, C-HA tag||HG11101-CY|
|Human SLAMF8 Gene cDNA clone plasmid||HG11101-M|
|Human SLAMF8 ORF mammalian expression plasmid, Flag tag||HG11101-M-F|
|Human SLAMF8 ORF mammalian expression plasmid, N-Flag tag||HG11101-NF|
|Human SLAMF8 ORF mammalian expression plasmid, N-His tag||HG11101-NH|
|Human SLAMF8 ORF mammalian expression plasmid, N-Myc tag||HG11101-NM|
|Human SLAMF8 ORF mammalian expression plasmid, N-HA tag||HG11101-NY|
|Human SLAMF8 natural ORF mammalian expression plasmid||HG11101-UT|
|Learn more about expression Vectors|
The signaling lymphocyte activation molecule (SLAM) family includes homophilic and heterophilic receptors that modulate both adaptive and innate immune responses. These receptors share a common ectodomain organization: a membrane-proximal immunoglobulin constant domain and a membrane-distal immunoglobulin variable domain that is responsible for ligand recognition. SLAM family of receptors is expressed by a wide range of immune cells. Through their cytoplasmic domain, SLAM family receptors associate with SLAM-associated protein (SAP)-related molecules, a group of cytoplasmic adaptors composed almost exclusively of an SRC homology 2 domain. SLAM family receptors, in association with SAP family adaptors, have crucial roles during normal immune reactions in innate and adaptive immune cells.
Mouse SLAM family member 8, also known as B-lymphocyte activator macrophage expressed, BCM-like membrane protein, SLAMF8 and BLAME, is a single-pass type I membrane protein. It contains one Ig-like C2-type (immunoglobulin-like) domain. SLAMF8 / BLAME is expressed in lymph node, spleen, thymus and bone marrow. It may play a role in B-lineage commitment and/or modulation of signaling through the B-cell receptor.