|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.
Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokarfyotic expression systems.
|Human CXCL8 ORF mammalian expression plasmid, C-GFPSpark tag||HG10098-ACG|
|Human CXCL8 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10098-ACR|
|Human CXCL8 ORF mammalian expression plasmid, C-Flag tag||HG10098-CF|
|Human CXCL8 ORF mammalian expression plasmid, C-His tag||HG10098-CH|
|Human CXCL8 ORF mammalian expression plasmid, C-Myc tag||HG10098-CM|
|Human CXCL8 ORF mammalian expression plasmid, C-HA tag||HG10098-CY|
|Human CXCL8 Gene cDNA clone plasmid||HG10098-M|
|Human CXCL8 ORF mammalian expression plasmid, N-Flag tag||HG10098-NF|
|Human CXCL8 ORF mammalian expression plasmid, N-His tag||HG10098-NH|
|Human CXCL8 ORF mammalian expression plasmid, N-Myc tag||HG10098-NM|
|Human CXCL8 ORF mammalian expression plasmid, N-HA tag||HG10098-NY|
|Human CXCL8 natural ORF mammalian expression plasmid||HG10098-UT|
|Learn more about expression Vectors|
Interleukin 8 (IL-8), also known as CXCL8, which is a chemokine with a defining CXC amino acid motif that was initially characterized for its leukocyte chemotactic activity, is now known to possess tumorigenic and proangiogenic properties as well. This chemokine is secreted by a variety of cell types including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, and various tumor cell lines in response to inflammatory stimuli (IL1, TNF, LPS, etc). In human gliomas, IL-8 is expressed and secreted at high levels both in vitro and in vivo, and recent experiments suggest it is critical to glial tumor neovascularity and progression. Levels of IL-8 correlate with histologic grade in glial neoplasms, and the most malignant form, glioblastoma, shows the highest expression in pseudopalisading cells around necrosis, suggesting that hypoxia/anoxia may stimulate expression. Interleukin (IL)-8/CXCL8 is a potent neutrophil chemotactic factor. Accumulating evidence has demonstrated that various types of cells can produce a large amount of IL-8/CXCL8 in response to a wide variety of stimuli, including proinflammatory cytokines, microbes and their products, and environmental changes such as hypoxia, reperfusion, and hyperoxia. Numerous observations have established IL-8/CXCL8 as a key mediator in neutrophil-mediated acute inflammation due to its potent actions on neutrophils. However, several lines of evidence indicate that IL-8/CXCL8 has a wide range of actions on various types of cells, including lymphocytes, monocytes, endothelial cells, and fibroblasts, besides neutrophils. The discovery of these biological functions suggests that IL-8/CXCL8 has crucial roles in various pathological conditions such as chronic inflammation and cancer. IL-8 has been associated with tumor angiogenesis, metastasis, and poor prognosis in breast cancer. IL-8 may present a novel therapeutic target for estrogen driven breast carcinogenesis and tumor progression.