|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human SECTM1 ORF mammalian expression plasmid, C-GFPSpark tag||HG12269-ACG|
|Human SECTM1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG12269-ACR|
|Human SECTM1 ORF mammalian expression plasmid, C-Flag tag||HG12269-CF|
|Human SECTM1 ORF mammalian expression plasmid, C-His tag||HG12269-CH|
|Human SECTM1 ORF mammalian expression plasmid, C-Myc tag||HG12269-CM|
|Human SECTM1 ORF mammalian expression plasmid, C-HA tag||HG12269-CY|
|Human SECTM1 Gene cDNA clone plasmid||HG12269-G|
|Human SECTM1 ORF mammalian expression plasmid, N-Flag tag||HG12269-NF|
|Human SECTM1 ORF mammalian expression plasmid, N-His tag||HG12269-NH|
|Human SECTM1 ORF mammalian expression plasmid, N-Myc tag||HG12269-NM|
|Human SECTM1 ORF mammalian expression plasmid, N-HA tag||HG12269-NY|
|Human SECTM1 natural ORF mammalian expression plasmid||HG12269-UT|
|Learn more about expression Vectors|
Secreted and transmembrane 1 (SECTM1), also known as K12, is a transmembrane and secreted protein with characteristics of a type 1a transmembrane protein of SECTM family. It is found in a perinuclear Golgi-like pattern and thought to be involved in hematopoietic and/or immune system processes. The human K12 protein has been shown to be primarily expressed in spleen, prostate, testis, small intestine, and in peripheral blood leukocytes. The K12 protein is expressed on the cell surface in such small amounts as to preclude detection. Alternatively, it may be that K12 on the cell surface is rapidly cleaved to generate a soluble K12 protein. Immunohistochemical analysis of peripheral blood cells shows that K12 is found in leukocytes of the myeloid lineage, with the strongest staining observed in granulocytes and no detectable expression in lymphocytes. May be involved in thymocyte signaling. It had been suggested a role for thymic microenvironment-produced K12 in regulation of thymocyte signaling and cytokine release, particularly in the setting of thymus pathology where IFN-gamma is upregulated such as myasthenia gravis. In addition, as a putative natural CD7 ligand, SECTM1/K12 may be responsible for the costimulatory role it plays in T cell activation.