|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human SRPK1 ORF mammalian expression plasmid, C-GFPSpark tag||HG12249-ACG|
|Human SRPK1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG12249-ACR|
|Human SRPK1 ORF mammalian expression plasmid, N-GFPSpark tag||HG12249-ANG|
|Human SRPK1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG12249-ANR|
|Human SRPK1 ORF mammalian expression plasmid, C-Flag tag||HG12249-CF|
|Human SRPK1 ORF mammalian expression plasmid, C-His tag||HG12249-CH|
|Human SRPK1 ORF mammalian expression plasmid, C-Myc tag||HG12249-CM|
|Human SRPK1 ORF mammalian expression plasmid, C-HA tag||HG12249-CY|
|Human SRPK1 Gene cDNA clone plasmid||HG12249-G|
|Human SRPK1 ORF mammalian expression plasmid, N-Flag tag||HG12249-NF|
|Human SRPK1 ORF mammalian expression plasmid, N-His tag||HG12249-NH|
|Human SRPK1 ORF mammalian expression plasmid, N-Myc tag||HG12249-NM|
|Human SRPK1 ORF mammalian expression plasmid, N-HA tag||HG12249-NY|
|Human SRPK1 natural ORF mammalian expression plasmid||HG12249-UT|
|Learn more about expression Vectors|
Serine / threonine-protein kinase SRPK1, also known as SFRS protein kinase 1, Serine/arginine-rich protein-specific kinase 1, SR-protein-specific kinase 1 and SRPK1, is a cytoplasm and nucleus protein which belongs to the protein kinase superfamily and CMGC Ser/Thr protein kinase family. Isoform 2 of SRPK1 is predominantly expressed in the testis but is also present at lower levels in heart, ovary, small intestine, liver, kidney, pancreas and skeletal muscle. Isoform 1 of SRPK1 is only seen in the testis, at lower levels than isoform 2. SRPK1 hyperphosphorylates RS domain-containing proteins such as SFRS1, SFRS2 and ZRSR2 on serine residues during metaphase but at lower levels during interphase. SRPK1 plays a central role in the regulatory network for splicing, controlling the intranuclear distribution of splicing factors in interphase cells and the reorganization of nuclear speckles during mitosis. SRPK1 locks onto SFRS1 to form a stable complex and processively phosphorylates the RS domain. SRPK1 appears to mediate HBV core protein phosphorylation which is a prerequisite for pregenomic RNA encapsidation into viral capsids.