|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human CCDC134 ORF mammalian expression plasmid, C-GFPSpark tag||HG13168-ACG|
|Human CCDC134 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13168-ACR|
|Human CCDC134 ORF mammalian expression plasmid, C-Flag tag||HG13168-CF|
|Human CCDC134 ORF mammalian expression plasmid, C-His tag||HG13168-CH|
|Human CCDC134 ORF mammalian expression plasmid, C-Myc tag||HG13168-CM|
|Human CCDC134 ORF mammalian expression plasmid, C-HA tag||HG13168-CY|
|Human CCDC134 Gene cDNA clone plasmid||HG13168-G|
|Human CCDC134 ORF mammalian expression plasmid, N-Flag tag||HG13168-NF|
|Human CCDC134 ORF mammalian expression plasmid, N-His tag||HG13168-NH|
|Human CCDC134 ORF mammalian expression plasmid, N-Myc tag||HG13168-NM|
|Human CCDC134 ORF mammalian expression plasmid, N-HA tag||HG13168-NY|
|Human CCDC134 natural ORF mammalian expression plasmid||HG13168-UT|
|Learn more about expression Vectors|
Human coiled-coil domain containing 134 (CCDC134) is a 229 amino acids secretory protein. Coiled-coil domain is a motif in which alpha-helix are coiled together. It has been found in many types of proteins, including transcription factors, intermediate filaments and certain tRNA synthetases. Many proteins containing such motif CCDC134 are involved in important biological functions. CCDC134 is also considered as a novel human MAPK-regulating protein that can inhibit the MAPK pathway. This protein significantly inhibite Elk1 transcriptional activity. The coiled-coil domain is a ubiquitous protein motif that is often involved in oligomerization.