|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human CAMKV ORF mammalian expression plasmid, C-GFPSpark tag||HG12243-ACG|
|Human CAMKV ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG12243-ACR|
|Human CAMKV ORF mammalian expression plasmid, N-GFPSpark tag||HG12243-ANG|
|Human CAMKV ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG12243-ANR|
|Human CAMKV ORF mammalian expression plasmid, C-Flag tag||HG12243-CF|
|Human CAMKV ORF mammalian expression plasmid, C-His tag||HG12243-CH|
|Human CAMKV ORF mammalian expression plasmid, C-Myc tag||HG12243-CM|
|Human CAMKV ORF mammalian expression plasmid, C-HA tag||HG12243-CY|
|Human CAMKV Gene cDNA clone plasmid||HG12243-G|
|Human CAMKV ORF mammalian expression plasmid, N-Flag tag||HG12243-NF|
|Human CAMKV ORF mammalian expression plasmid, N-His tag||HG12243-NH|
|Human CAMKV ORF mammalian expression plasmid, N-Myc tag||HG12243-NM|
|Human CAMKV ORF mammalian expression plasmid, N-HA tag||HG12243-NY|
|Human CAMKV natural ORF mammalian expression plasmid||HG12243-UT|
|Learn more about expression Vectors|
CaM kinase-like vesicle-associated protein, also known as CAMKV, is a peripheral membrane protein and Cytoplasmic vesicle membrane protein which belongs to the protein kinase superfamily and CAMK Ser/Thr protein kinase family. CAMKV contains one protein kinase domain. It is predominantly observed in association with the plasma membrane of soma and in neurites, both axons and dendrites. CAMKV may be associated with vesicular structures. It does not appear to have detectable kinase activity.
Protein kinases are a group of enzymes that move a phosphate group onto proteins, in a process called phosphorylation. Protein kinases function as an on/off switch for many cellular processes, including metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. They also function in embryonic development, physiological responses, and in the nervous and immune system. Abnormal phosphorylation causes many human diseases, including cancer, and drugs that affect phosphorylation can treat those diseases. The protein kinase domain is a structurally conserved protein domain containing the catalytic function of protein kinases. Protein kinases play a role in a mulititude of cellular processes, including division, proliferation, apoptosis, and differentiation. Phosphorylation usually results in a functional change of the target protein by changing enzyme activity, cellular location, or association with other proteins.