|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human AK2 ORF mammalian expression plasmid, C-GFPSpark tag||HG13146-ACG|
|Human AK2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13146-ACR|
|Human AK2 ORF mammalian expression plasmid, N-GFPSpark tag||HG13146-ANG|
|Human AK2 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG13146-ANR|
|Human AK2 ORF mammalian expression plasmid, C-Flag tag||HG13146-CF|
|Human AK2 ORF mammalian expression plasmid, C-His tag||HG13146-CH|
|Human AK2 ORF mammalian expression plasmid, C-Myc tag||HG13146-CM|
|Human AK2 ORF mammalian expression plasmid, C-HA tag||HG13146-CY|
|Human AK2 Gene cDNA clone plasmid||HG13146-G|
|Human AK2 ORF mammalian expression plasmid, N-Flag tag||HG13146-NF|
|Human AK2 ORF mammalian expression plasmid, N-His tag||HG13146-NH|
|Human AK2 ORF mammalian expression plasmid, N-Myc tag||HG13146-NM|
|Human AK2 ORF mammalian expression plasmid, N-HA tag||HG13146-NY|
|Human AK2 natural ORF mammalian expression plasmid||HG13146-UT|
|Learn more about expression Vectors|
Adenylate kinase 2 (AK2) belongs to the Adenylate kinase family that contains three isozymes: AK1, AK2 and AK3. Adenylate kinases are involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. Adenylate kinase2 (AK2) is expressed in mitochondrial intermembrane space. It may play a role in apoptosis. It has been demonstrated that in apoptotic cells AK2 was translocated into the cytosol concomitantly with cytochronme C. Mutations in this gene are the cause of reticular dysgenesis. These mutations result in absent or strongly decreased protein expression. It has been also established that AK2 is specifically expressed in the stria vascularis region of the inner ear, which provides an explanation of the sensorineural deafness in these individuals.