|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human TNFSF4 ORF mammalian expression plasmid, C-GFPSpark tag||HG13127-ACG|
|Human TNFSF4 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13127-ACR|
|Human TNFSF4 ORF mammalian expression plasmid, C-Flag tag||HG13127-CF|
|Human TNFSF4 ORF mammalian expression plasmid, C-His tag||HG13127-CH|
|Human TNFSF4 ORF mammalian expression plasmid, C-Myc tag||HG13127-CM|
|Human TNFSF4 ORF mammalian expression plasmid, C-HA tag||HG13127-CY|
|Human TNFSF4 Gene cDNA clone plasmid||HG13127-G|
|Human TNFSF4 ORF mammalian expression plasmid, N-Flag tag||HG13127-NF|
|Human TNFSF4 ORF mammalian expression plasmid, N-His tag||HG13127-NH|
|Human TNFSF4 ORF mammalian expression plasmid, N-Myc tag||HG13127-NM|
|Human TNFSF4 ORF mammalian expression plasmid, N-HA tag||HG13127-NY|
|Human TNFSF4 natural ORF mammalian expression plasmid||HG13127-UT|
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OX-40L, also known as TNFSF4 and CD252, is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. OX-40L is an important costimulatory molecule that plays a crucial role in the regulation of T-cell-mediated immunity. The interaction of TNFSF4-TNFSF4 is involved in the pathogenesis of multiple autoimmune and inflammatory diseases such as systemic lupus erythematosus (SLE), carotid artery disease and cancer. OX-40L is a ligand for receptor TNFRSF4/OX4. It is found to play a role in T cell antigen-presenting cell (APC) interactions. In surface Ig- and CD40-stimulated B cells, this cytokine along with CD70 has been shown to provide CD28-independent costimulatory signals to T cells. This protein and its receptor are reported to directly mediate adhesion of activated T cells to vascular endothelial cells.