|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human SERPINA6 ORF mammalian expression plasmid, C-GFPSpark tag||HG10998-ACG|
|Human SERPINA6 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10998-ACR|
|Human SERPINA6 ORF mammalian expression plasmid, C-Flag tag||HG10998-CF|
|Human SERPINA6 ORF mammalian expression plasmid, C-His tag||HG10998-CH|
|Human SERPINA6 ORF mammalian expression plasmid, C-Myc tag||HG10998-CM|
|Human SERPINA6 ORF mammalian expression plasmid, C-HA tag||HG10998-CY|
|Human SERPINA6 Gene cDNA clone plasmid||HG10998-M|
|Human SERPINA6 ORF mammalian expression plasmid, Flag tag||HG10998-M-F|
|Human SERPINA6 ORF mammalian expression plasmid, N-Flag tag||HG10998-NF|
|Human SERPINA6 ORF mammalian expression plasmid, N-His tag||HG10998-NH|
|Human SERPINA6 ORF mammalian expression plasmid, N-Myc tag||HG10998-NM|
|Human SERPINA6 ORF mammalian expression plasmid, N-HA tag||HG10998-NY|
|Human SERPINA6 natural ORF mammalian expression plasmid||HG10998-UT|
|Learn more about expression Vectors|
Corticosteroid-binding globulin (CBG), also known as SerpinA6, is a non-inhibitory member of the serine proteinase inhibitor (serpin) superfamily. It is the high-affinity transport protein for glucocorticoids in vertebrate blood. CBG is specifically cleaved by this protease at a precise site close to its carboxy-terminus. This induces a conformation change and disrupts the binding between glucocorticoids and CBG, and promotes a significant and local release of glucocorticoids (over 90% of them are bound to CBG in human plasma). In this context, CBG directs glucocorticoids to sites of inflammation, and plays in consequence a crucial role in efficient glucocorticoid action in physiology. The SerpinA6 protein is mainly secreted by the liver. This negative acute phase protein regulates free cortisol levels in the blood and distributes cortisol to its target tissues. SerpinA6 deficiency is an extremely rare hereditary disorder characterized by reduced corticosteroid-binding capacity with normal or low plasma corticosteroid-binding globulin concentration, and normal or low basal cortisol levels associated with hypo-/hypertension and muscle fatigue. There are three heritable, human CBG gene mutations that can reduce CBG-cortisol binding affinity and/or reduce circulating CBG levels.