|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human IL6ST ORF mammalian expression plasmid, C-GFPSpark tag||HG10974-ACG|
|Human IL6ST ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10974-ACR|
|Human IL6ST ORF mammalian expression plasmid, C-Flag tag||HG10974-CF|
|Human IL6ST ORF mammalian expression plasmid, C-His tag||HG10974-CH|
|Human IL6ST ORF mammalian expression plasmid, C-Myc tag||HG10974-CM|
|Human IL6ST ORF mammalian expression plasmid, C-HA tag||HG10974-CY|
|Human IL6ST Gene cDNA clone plasmid||HG10974-M|
|Human IL6ST ORF mammalian expression plasmid, N-Flag tag||HG10974-NF|
|Human IL6ST ORF mammalian expression plasmid, N-His tag||HG10974-NH|
|Human IL6ST ORF mammalian expression plasmid, N-Myc tag||HG10974-NM|
|Human IL6ST ORF mammalian expression plasmid, N-HA tag||HG10974-NY|
|Human IL6ST natural ORF mammalian expression plasmid||HG10974-UT|
|Learn more about expression Vectors|
Glycoprotein 130 (also known as gp130, IL6ST, IL6-beta or CD130) is a transmembrane protein which is the founding member of the class of all cytokine receptors. CD130/gp130 is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and Oncostatin M (OSM). CD130/gp130 functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. CD130/gp130 plays a critical role in regulating myocyte apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been described. A related pseudogene has been identified on chromosome 17. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. CD130/gp130 binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. CD130/gp130 may have a role in embryonic development. The type I OSM receptor is capable of transducing OSM-specific signaling events.