|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human AARS ORF mammalian expression plasmid, C-GFPSpark tag||HG10951-ACG|
|Human AARS ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10951-ACR|
|Human AARS ORF mammalian expression plasmid, N-GFPSpark tag||HG10951-ANG|
|Human AARS ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG10951-ANR|
|Human AARS ORF mammalian expression plasmid, C-Flag tag||HG10951-CF|
|Human AARS ORF mammalian expression plasmid, C-His tag||HG10951-CH|
|Human AARS ORF mammalian expression plasmid, C-Myc tag||HG10951-CM|
|Human AARS ORF mammalian expression plasmid, C-HA tag||HG10951-CY|
|Human AARS Gene cDNA clone plasmid||HG10951-M|
|Human AARS natural ORF mammalian expression plasmid||HG10951-M-N|
|Human AARS ORF mammalian expression plasmid, N-Flag tag||HG10951-NF|
|Human AARS ORF mammalian expression plasmid, N-His tag||HG10951-NH|
|Human AARS ORF mammalian expression plasmid, N-Myc tag||HG10951-NM|
|Human AARS ORF mammalian expression plasmid, N-HA tag||HG10951-NY|
|Human AARS natural ORF mammalian expression plasmid||HG10951-UT|
|Learn more about expression Vectors|
Alanyl-tRNA synthetase (AARS) belongs to the family of ligases, specifically those forming carbon-oxygen bonds in aminoacyl-tRNA and related compounds. This enzyme participates in alanine and aspartate metabolism and aminoacyl-tRNA biosynthesis. Alanyl-tRNA synthetase (AlaRS) catalyzes synthesis of Ala-tRNA (Ala) and hydrolysis of mis-acylated Ser- and Gly-tRNA (Ala) at 2 different catalytic sites. Their role is not confined to catalyze the attachment of amino acids to transfer RNAs and thereby establish the rules of genetic code by virtue of matching the nucleotide triplet of anticodon with cognate amino acid. Under apoptotic conditions in cell culture, the full-length enzyme is secreted, and the two cytokine activities can be generated by leukocyte elastase, an extracellular protease. Secretion of this tRNA synthetase may contribute to apoptosis both by arresting translation and producing needed cytokines. This protein could be an attractive target of drugs against bacterial, fungal and parasitic infections.