|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human EZR ORF mammalian expression plasmid, C-GFPSpark tag||HG12858-ACG|
|Human EZR ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG12858-ACR|
|Human EZR ORF mammalian expression plasmid, C-Flag tag||HG12858-CF|
|Human EZR ORF mammalian expression plasmid, C-His tag||HG12858-CH|
|Human EZR ORF mammalian expression plasmid, C-Myc tag||HG12858-CM|
|Human EZR ORF mammalian expression plasmid, C-HA tag||HG12858-CY|
|Human EZR Gene cDNA clone plasmid||HG12858-G|
|Human EZR ORF mammalian expression plasmid, N-Flag tag||HG12858-NF|
|Human EZR ORF mammalian expression plasmid, N-His tag||HG12858-NH|
|Human EZR ORF mammalian expression plasmid, N-Myc tag||HG12858-NM|
|Human EZR ORF mammalian expression plasmid, N-HA tag||HG12858-NY|
|Human EZR natural ORF mammalian expression plasmid||HG12858-UT|
|Learn more about expression Vectors|
VIL2, also known as villin-2, belongs to the ERM protein family. It is expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. VIL2 contains 1 FERM domain and may be involved in connections of major cytoskeletal structures to the plasma membrane. It plays a key role in cell surface structure adhesion, migration, and organization. In epithelial cells, VIL2 is required for the formation of microvilli and membrane ruffles on the apical pole. It interacts with MPP5 and SLC9A3R2. It is found VIL2 form a complex with PODXL and SLC9A3R2. It also interacts with MCC, PLEKHG6, PODXL, SCYL3/PACE1, SLC9A3R1 and TMEM8B.